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Quantification of malate content in CBP lines. a) Malate content per milligram fresh weight (FW) of <t>titratable</t> acid analysis of CK and CBP lines at 12:00 am (night) and 12:00 pm (day). n = 3. b) Determine the relative malate content per milligram of fresh weight (FW) in HPLC‐MS analysis of the CK and CBP lines at 12:00 am (night) and 12:00 pm (day). n = 3. c) The images show emission intensities of protoplast vacuoles in rice loaded with BCECF at 488 nm (the first column, red) and 458 nm (the second column, green). The ratio images indicate an increased or decreased vacuolar pH in rice protoplasts. The pseudo‐colored scale below indicates the fluorescence intensity. Scale bar, 10 µm. d) Quantification of the luminal pH in rice protoplasts. Bars represent from six different intact vacuoles. Asterisks represent significant differences according to Student's t test, * p < 0.05, ** p < 0.01, *** p < 0.001. Note: Metabolite concentrations were reported as concentrations relative to the internal standard, which is the target compound peak area divided by the peak area of 2‐Aminobutyric acid: N (relative concentration)= Xi (target compound peak arc(a) *X' IS (peak area of 2‐Aminobutyrie acid per gram fresh weight.
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Quantification of malate content in CBP lines. a) Malate content per milligram fresh weight (FW) of <t>titratable</t> acid analysis of CK and CBP lines at 12:00 am (night) and 12:00 pm (day). n = 3. b) Determine the relative malate content per milligram of fresh weight (FW) in HPLC‐MS analysis of the CK and CBP lines at 12:00 am (night) and 12:00 pm (day). n = 3. c) The images show emission intensities of protoplast vacuoles in rice loaded with BCECF at 488 nm (the first column, red) and 458 nm (the second column, green). The ratio images indicate an increased or decreased vacuolar pH in rice protoplasts. The pseudo‐colored scale below indicates the fluorescence intensity. Scale bar, 10 µm. d) Quantification of the luminal pH in rice protoplasts. Bars represent from six different intact vacuoles. Asterisks represent significant differences according to Student's t test, * p < 0.05, ** p < 0.01, *** p < 0.001. Note: Metabolite concentrations were reported as concentrations relative to the internal standard, which is the target compound peak area divided by the peak area of 2‐Aminobutyric acid: N (relative concentration)= Xi (target compound peak arc(a) *X' IS (peak area of 2‐Aminobutyrie acid per gram fresh weight.
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Quantification of malate content in CBP lines. a) Malate content per milligram fresh weight (FW) of <t>titratable</t> acid analysis of CK and CBP lines at 12:00 am (night) and 12:00 pm (day). n = 3. b) Determine the relative malate content per milligram of fresh weight (FW) in HPLC‐MS analysis of the CK and CBP lines at 12:00 am (night) and 12:00 pm (day). n = 3. c) The images show emission intensities of protoplast vacuoles in rice loaded with BCECF at 488 nm (the first column, red) and 458 nm (the second column, green). The ratio images indicate an increased or decreased vacuolar pH in rice protoplasts. The pseudo‐colored scale below indicates the fluorescence intensity. Scale bar, 10 µm. d) Quantification of the luminal pH in rice protoplasts. Bars represent from six different intact vacuoles. Asterisks represent significant differences according to Student's t test, * p < 0.05, ** p < 0.01, *** p < 0.001. Note: Metabolite concentrations were reported as concentrations relative to the internal standard, which is the target compound peak area divided by the peak area of 2‐Aminobutyric acid: N (relative concentration)= Xi (target compound peak arc(a) *X' IS (peak area of 2‐Aminobutyrie acid per gram fresh weight.
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Quantification of malate content in CBP lines. a) Malate content per milligram fresh weight (FW) of titratable acid analysis of CK and CBP lines at 12:00 am (night) and 12:00 pm (day). n = 3. b) Determine the relative malate content per milligram of fresh weight (FW) in HPLC‐MS analysis of the CK and CBP lines at 12:00 am (night) and 12:00 pm (day). n = 3. c) The images show emission intensities of protoplast vacuoles in rice loaded with BCECF at 488 nm (the first column, red) and 458 nm (the second column, green). The ratio images indicate an increased or decreased vacuolar pH in rice protoplasts. The pseudo‐colored scale below indicates the fluorescence intensity. Scale bar, 10 µm. d) Quantification of the luminal pH in rice protoplasts. Bars represent from six different intact vacuoles. Asterisks represent significant differences according to Student's t test, * p < 0.05, ** p < 0.01, *** p < 0.001. Note: Metabolite concentrations were reported as concentrations relative to the internal standard, which is the target compound peak area divided by the peak area of 2‐Aminobutyric acid: N (relative concentration)= Xi (target compound peak arc(a) *X' IS (peak area of 2‐Aminobutyrie acid per gram fresh weight.

Journal: Advanced Science

Article Title: A Synthetic Facultative CAM‐Like Shuttle in C 3 Rice Plants

doi: 10.1002/advs.202500418

Figure Lengend Snippet: Quantification of malate content in CBP lines. a) Malate content per milligram fresh weight (FW) of titratable acid analysis of CK and CBP lines at 12:00 am (night) and 12:00 pm (day). n = 3. b) Determine the relative malate content per milligram of fresh weight (FW) in HPLC‐MS analysis of the CK and CBP lines at 12:00 am (night) and 12:00 pm (day). n = 3. c) The images show emission intensities of protoplast vacuoles in rice loaded with BCECF at 488 nm (the first column, red) and 458 nm (the second column, green). The ratio images indicate an increased or decreased vacuolar pH in rice protoplasts. The pseudo‐colored scale below indicates the fluorescence intensity. Scale bar, 10 µm. d) Quantification of the luminal pH in rice protoplasts. Bars represent from six different intact vacuoles. Asterisks represent significant differences according to Student's t test, * p < 0.05, ** p < 0.01, *** p < 0.001. Note: Metabolite concentrations were reported as concentrations relative to the internal standard, which is the target compound peak area divided by the peak area of 2‐Aminobutyric acid: N (relative concentration)= Xi (target compound peak arc(a) *X' IS (peak area of 2‐Aminobutyrie acid per gram fresh weight.

Article Snippet: Malate content was determined using the Solarbio (China) Titratable Acid (TA) Detection Kit (via titration method) according to the kit instructions.

Techniques: Fluorescence, Concentration Assay